KMID : 1377020180150010001
|
|
Tissue Engineering and Regenerative Medicine 2018 Volume.15 No. 1 p.1 ~ p.11
|
|
Oral Immunization of FMDV Vaccine Using pH-Sensitive and Mucoadhesive Thiolated Cellulose Acetate Phthalate Microparticles
|
|
Lee Ho-Bin
Yoon So-Yeon Singh Bijay Oh Seo-Ho Cui Lianhua Yan Changguo Kang Sang-Kee Choi Yun-Jaie Cho Chong-Su
|
|
Abstract
|
|
|
Several barriers such as gastric pH, enzymatic degradation and rapid transit should be overcome to orally deliver antigens for taking up by epithelial microfold cells in Peyer¡¯s patches of small intestine. To solve the above mentioned problems, we designed pH-sensitive and mucoadhesive polymeric microparticles (MPs) prepared by double emulsion technique using cellulose acetate phthalate (CAP) to enhance immune response of foot-and-mouth disease (FMD) virus (FMDV) subunit vaccine. Thiolation of CAP improved mucoadhesive property of CAP to prolong the MPs transit time through the gastrointestinal tract. Thiolated CAP (T-CAP) also slowed down antigen release in acidic pH of stomach but released more antigens in neutral pH of small intestine due to the pH-sensitivity of the T-CAP. Oral immunization of a chimerical multi-epitope recombinant protein as the FMD subunit vaccine via T-CAP MPs effectively delivered the vaccine to Peyer¡¯s patches eliciting mucosal IgA response. It will make a step forward into a promising oral subunit vaccine development in livestock industry.
|
|
KEYWORD
|
|
pH-senstive, Mucoadhesive, Foot-and-mouth disease, Subunit vaccine, Oral immunization
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|